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Localization of PilA1 in wild-type and pilA mutant strains of E. corrodens. (A) Total protein fraction; (B) surface protein fraction. Purified PilA1 (lane 1) and protein fractions from strains VA1-S1 (lane 2), VA1-L2 (lane 3), T18 (lane 4), T6 (lane 5), T11 (lane 6), T40 (lane 7), and T99 (lane 8) were subjected to immunoblot analysis with a polyclonal antiserum specific for PilA1. Each arrow marks the position of mature pilin.
