FIG. 5.

Physical maps and corresponding phenotypes of examined wild-type and pilA mutant strains of E. corrodens. Strains VA1-S1 and VA1-L2 are S-phase and L-phase variants, respectively, of the clinical isolate VA1. Mutant strains T18, T6, T11, T40, and T99 were isolated following transformation of strain VA1-S1 with the corresponding plasmid and selecting for double homologous recombinants. Each strain was assayed for colony morphology, surface pili, PilA1, competence for natural transformation, and twitching motility as described in Materials and Methods. Symbols indicate a positive (+) or negative (−) assay result.