TABLE 2.
Primers used
| Primera | 5′→3′ sequenceb | Position,c restriction site |
|---|---|---|
| RH-1 (←) | TTCGggAtCcTTAGCAGCACCAGGAGCG | 888–915, BamHI site at 892 |
| RH-2 (←) | GGCAACTTGATGGCAAATATCCTAC | 1431–1454 |
| RH-3 (→) | GGCACCCAAACCCTTTACAAG | 1590–1610 |
| RH-10 (→) | CGGTCAGATCTCTACTTGGACTTGCGC | 864–890, BglII site at 869 |
| RH-7B (←) | GATAGgGATCcGCCTGCATGGAAGGGG | 1192–2018, BamHI site at 2008 |
| RH-8 (←) | CCGGTCgGATCcCTACTTGGACTTGC | 863–888, BamHI site at 868 |
| RH-9 (←) | GATAGAGATCTGCCTGCATGGAAGG | 1994–2018, BglII site at 2008 |
| RH-18 (→) | AGATCCCgggTGTTTGGCAAGGGGGAT | 967–993, SmaI site at 971 |
| RH-12(←) | AGCTTctcgaGGCAGAAACTATCTGCCTGC | 2128–2158, XhoI site at 2133 |
| RH-14 (←) | ATTCActCGaGCTTTTTCGCCAACATCGTC | 3299–3328, XhoI site at 3318 |
| 105-R1 (→) | TGTTATCGCCATTATCGG | 531–548 |
| 107-F3 (→) | AGAGCAACTCGCTTTACCC | 1079–1096 |
| 204-F2 (→) | CGGATACGATGTGCATG | 2885–2901 |
a
Arrows designate forward (→) or reverse (←) primer sequence.
b
Underscored letters correspond to specified restriction sites; lowercase letters designate introduced base substitutions.
c
Nucleotide numbers corresponding to pilA locus sequence deposited in GenBank (accession no. AF079304).