Figure 4.

Oxaliplatin-treated primary laryngeal cancer cells pulsed with DCs induced the populations of tumor-specific CD8⁺ T cells and reduced the population of Treg cells. Cisplatin or oxaliplatin-treated primary laryngeal cancer cells were pulsed with monocyte-derived DCs. After that, the cells were used to stimulate autologous T cells for 2 weeks. (a-b) The numbers of IFN-γ-producing CD8⁺ T cells were analyzed by intracellular IFN-γ staining after stimulation. The frequencies of CD4⁺CD25⁺FoxP3⁺ Treg cells were analyzed by a flow cytometer. The results are from five independent studies. Data were represented as the means ± SD. ^∗∗p < 0.01 compared with the cisplatin group.