Table 2.
Primers used in RT-PCR.
| Gene | Primer (5′→3′) |
|---|---|
| Nrf2 | Forward: GAGAGCCCAGTCTTCATTGC Reverse: TTGGCTTCTGGACTTGGAAC |
| Keap1 | Forward: TTCAAGGCCATGTTCACCAA |
| Reverse: TGGATACCCTCAATGGACACC | |
| NQO1 | Forward: GGAGAGTTTGCTTACACTTACGC |
| Reverse: AGTGGTGATGGAAAGCACTGCCTTC | |
| HO-1 | Forward: CGCCTTCCTGCTCAACATT |
| Reverse: TGTGTTCCTCTGTCAGCATCAC | |
| KLF9 | Forward: GGGAAACCTCCGAAAA |
| Reverse: CGTTCACCTGTATGCACTGTA | |
| GAPDH | Forward: GGAGATTACTGCCCTGGCTC Reverse: GACTCATCGTACTCCT |
RT-PCR was performed on the ABI PRISM® 7500 real-time PCR analyzer (Applied Biosystems, Foster City, CA, USA) using the SYBR® Premix Ex Taq™ RT-PCR Kit. The relative mRNA expression was calculated by means of 2−ΔΔCt and was normalized to the mean mRNA expressions of GAPDH. The results were calculated with the following formulae: ratio = 2−ΔΔCt, ΔΔCt = (Cttarget − CtGAPDH)Sample − (Cttarget − CtGAPDH)Control.