Figure 5.
Absence of B0AT1 associated with basal albuminuria but mitigated aristolochic acid (AA)-induced albuminuria. A: the urinary albumin-to-creatinine ratio (UACR) under basal conditions (n = 18–28 mice/pooled groups before injection of vehicle or AA), after injections (INJ), and at harvest. Kidney mRNA expression of Lrp2 (megalin; B), and organic anion transporters Slc22a6 [organic anion transporter 1 (OAT1)] and Slc22a8 [organic anion transporter 3 (OAT3)] (C) in mice treated with vehicle or AA. mRNA expression was normalized to the ribosomal protein L19 (Rpl19) gene. Values are means ± SE and dots show individual mouse data. For basal conditions, one-way ANOVA was performed to probe for a significant effect of genotypes. For the other two time points and the mRNA expression data, two-way ANOVA was performed to probe for a significant effect of treatment (PAA), genotypes (PSlc6a19), or the interaction between the two factors (Pinter). If the interaction was statistically significant, then a pair-wise multiple comparison procedure (Holm–Sidak method) identified the significant effects. *P < 0.05 vs. wild-type (WT) mice; #P < 0.05 vs. vehicle. n = 6–14 mice/group.