Table 1.

Real-time PCR primer list

	Forward (5′-3′)	Reverse (5′-3′)
TRPA1	TCGTGTGAAGTGCTGAATAT	AACTTTACACTTTCAACTTGATTTT
TRPV1	TACTTTTCTTTGTACAGTCACT	TCAATCATGACAGCATAGAT
ASIC1	GCCTATGAGATCGCAGGG	AAAGTCCTCAAACGTGCCTC
ASIC2	GAAGAGGAAGGGAGCCATGAT	GGCAGAAGTTCGCAATGTGT
ASIC3	CCCAGCTCTGGACGCTATG	TCTTCCTGGAGCAGAGTGTTG
Piezo2	GCACTCTACCTCAGGAAGACTG	CAAAGCTGTGCCACCAGGTTCT
nNOS	TCAGTCTCCCAGGCTAATGG	CTGTCCACCTGGATTCCTGT
eNOS	GACCCTCACCGCTACAACAT	GCTCATTTTCCAGGTGCTTC
sGCα1	AGCGACTGAACCTTGCACTT	ACCTGCTGCAATTGCTTCTT
HIF-1α	TCAAGTCAGCAACGTGGAAG	TATCGAGGCTGTGTCGACTG
VEGF-A	CAGGCTGCTGTAACGATGAA	CAATTTGGCTCCTCCTACCA
TGF-β1	ACAGGGCTTTCGATTCAGCG	GGGGCTGATCCCGTTGATTTC
β-Actin	GCCCTGAGGCTCTTTTCCAG	TGCCACAGGATTCCATACCC

ASIC, acid-sensing ion channel; eNOS, endothelial nitric oxide synthase; HIF-1α, hypoxia-inducible factor-1α; nNOS, neuronal nitric oxide synthase; sGCα1, soluble guanylyl cyclase α1; TGF-β1, transforming growth factor-β1; TRPA1, transient receptor potential ankyrin 1; TRPV1, transient receptor potential vanilloid 1; VEGF-A, vascular endothelial growth factor A.