Figure 6.

Liddle mice excrete smaller urine volume and less urine Na⁺ in response to a saline load compared with wild-type (WT) or pseudohypoaldosteronism type 1 (PHA1) mice. A: experimental scheme. After male mice were fed a regular chow diet, they received infusion of desmopressin (dDAVP; 100 ng/h) for 2 days. Mice were then administered a saline load (8.4% NaCl in an intraperitoneal volume of 2.5% of body wt), and urine was collected over the ensuing 4 h. B: summary graphs of a timed (4-h) urine collection for means (±SE) urine flow rate, urine Na⁺ concentration (UNa⁺), and urine osmolality (urine osM) in WT, PHA1, and Liddle mice. C: summary graphs of a timed (4-h) urine collection for means (±SE) urine Na⁺ excretion (UNaV) and urine osmolar excretion (urine osM V) in WT, PHA1, and Liddle mice. *P < 0.05 by two-way ANOVA with Tukey correction from other groups of mice. n = 12 WT mice, 11 PHA1 mice, and 10 Liddle mice.