Figure 2.

Role of tissue-specific angiotensinogen (AGT) in 5/6 nephrectomy (Nx)-induced kidney and glomerular dysfunction. Kidney and blood samples were collected at 16 wk post-Nx. Mouse urine was collected during 12 h using metabolic cages. Plasma creatinine (PCr; A) and creatinine clearance (Ccr; B), calculated with the formulas described in materials and methods, were used as an index of kidney function (n = 6–10). C: urine albumin-to-creatinine ratio (UACR) was evaluated using ELISA as described in materials and methods and used as an index of glomerular function (n = 6–7). Data are expressed as means ± SD. **P < 0.01 vs. the respective sham; ***P < 0.001 vs. the respective sham; ##P < 0.01; ###P < 0.001. LKO, liver-specific AGT knockout; PKO, proximal tubule-specific AGT knockout; WT, wild type.