Figure 5.

Role of tissue-specific angiotensinogen (AGT) in 5/6 nephrectomy (Nx)-induced glomerulosclerosis. Kidney samples were collected at 16 wk post-Nx. A: paraffin-embedded kidney sections were used to carry out periodic acid-Schiff staining, Sirius red staining, and immunohistochemistry for evaluating the α-smooth muscle actin (α-SMA)-positive area. B: the glomerulosclerosis index was evaluated in at least 30 randomly chosen glomeruli per group, based on a modified semiquantitative scoring system as described in materials and methods. C and D: Sirius red- and α-SMA-positive areas were measured in at least 30 randomly chosen areas per kidney (n = 6–11). Scale bars = 50 µm. Data are expressed as means ± SD. ***P < 0.001 vs. the respective sham; #P < 0.05; ##P < 0.01; and ###P < 0.001. LKO, liver-specific AGT knockout; PKO, proximal tubule-specific AGT knockout; WT, wild type.