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. 2022 Aug 25;25(10):104971. doi: 10.1016/j.isci.2022.104971

Figure 3.

Figure 3

hPSC expansion in the thermoreversible hydrogel

(A) Experiment schematic of hPSC expansion for each passage. Ri = Rock Inhibitor (Y-27632). Further details in STAR Methods.

(B) Representative images of stem cell aggregates within the thermoreversible hydrogel by day 4 of each passage. All scale bars = 100 uM.

(C) hPSC fold change after 4 days of culture in each passage, between seeding count and final cell count. n = 3. Data are represented as mean +/− SD.

(D) PluriTest results of hPSCs maintained in the thermoreversible hydrogel for 5 passages.

(E) Representative images of hPSCs maintained for 5 passages in either the 3D thermoreversible hydrogel or 2D Matrigel. Scale bar = 100 μm. p < 0.05 was considered statistically significant (N.S. p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).