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. 2022 Sep 19;13(9):800. doi: 10.1038/s41419-022-05189-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A RT-qPCR analysis shows the expression levels of several miR-3940-3p target genes including KLLN in the TNBC cell lines and the normal breast epithelial cell line. B RT-qPCR analysis shows the expression levels of the miR-3940-3p target genes, BIRC5 and KLLN in the control, SEAS1-silenced, and SEAS1-overexpressing TNBC cell lines. C The wild-type and mutated binding sites of miR-3940-3p in the 3′-UTR of KLLN. D RT-qPCR analysis shows the results of anti-AGO2 RIP assay. The relative levels of KLLN mRNA pulled down from the MDA-MB-231cell extracts using anti-Ago2 antibody and IgG are shown with the input. E Luciferase reporter assays show the luciferase activity in MDA-MB-231 cells co-transfected with luciferase reporter vector cloned with the wild-type or mutant 3′UTR sequence of KLLN. F Western blot analysis shows the levels of KLLN protein in SEAS1-silenced TNBC cells co-transfected with miR-3940-3p inhibitor and the corresponding controls. G RT-qPCR analysis shows the levels of KLLN mRNA in SEAS1-silenced TNBC cells transfected with the miR-3940-3p inhibitor and the corresponding controls. H–J CCK8, colony formation, and EdU assay results show the proliferation rates of the TNBC cells co-transfected with miR-3940-3p inhibitor and siRNA against KLLN (si-KLLN) and the corresponding controls. K Transwell assay results show the migration rates of TNBC cells co-transfected with the miR-3940-3p inhibitor and si-KLLN and the corresponding controls. L Western blot results show the levels of caspase 3, caspase 7, BAX, PARP, and Bcl2 proteins in the TNBC cells co-transfected with the miR-3940-3p inhibitor and si-KLLN and the corresponding controls. M Western blot results show the levels of EMT-related proteins, namely, N-cadherin, Vimentin, Snail, and E-cadherin in TNBC cells co-transfected with the miR-3940-3p inhibitor and si-KLLN and the corresponding controls. *P < 0.05, **P < 0.01, and ***P < 0.001. Representative data from at least 3 experiments with comparable results is shown in the figure.