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. 2022 Sep 19;79(10):524. doi: 10.1007/s00018-022-04541-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The NF-kB inhibition induces apoptotic cell death in endometrioid cancer cells. A Ishikawa and AN3CA cells were treated with BAY11-7082 (NF-kB pathway inhibitor) for 48 h and cell viability was assessed by MTT assay. Similar results were obtained in three separate experiments. B Representative results of flow cytometry analysis of BAY11-7082-induced cell apoptosis. Ishikawa or AN3CA cells treated with BAY11-7082 10 µM for 48 h were stained with Annexin V and propidium iodide (left panel). Histograms represent the percentage of apoptotic cells (early, Q4 quadrant; and late apoptosis, Q2 quadrant), obtained in response to vehicle or BAY11-7082 treatment. Mean values ± SD of assays performed in duplicate are shown. C Expression of proteins from cells treated with NF-kB inhibitor BAY-118072 (10 µM, 48 h) was evaluated by immunoblotting. D Impairment of p65/RELA expression activates JNK apoptotic pathway. Immunoblot analysis of cells transiently transfected with an empty vector or a vector encoding for a non-degradable IKBα protein (SR-IKBα). Right histograms show the corresponding quantification of p65 levels, referred to GAPDH. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student’s t test)