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. 2001 Jan;183(1):77–85. doi: 10.1128/JB.183.1.77-85.2001

FIG. 5.

FIG. 5

Analysis of the effect of replacements in the ED(Y) motif of WbbE on O:54 LPS expression. Immunoblot of anti-O:54 reactive LPS from E. coli DH5α harboring wild-type or mutated WbbE and wild-type WbbF. Lanes: 1, pET30a(+) (negative control); 2, pWQ835; 3, pWQ835-180; 4, pWQ835-180, pWQ818; 5, pWQ835-180Q; 6, pWQ835-180Q, pWQ818; 7, pWQ835-181; 8, pWQ835-181A; 9, pWQ835-181A, pWQ818. R-LPS was not detected due to the use of antisera adsorbed against E. coli DH5α. The migration of S-LPS is indicated.