Fig. 1. Volumetric calcium imaging during a two-whisker task.

a Mice use two whiskers to detect a pole that appears in a proximal position range (light gray) or distal position (dark gray; Methods). Right, task timing. b Training progression (n = 7 mice). c Example touch video. Bottom left, the region where curvature is measured is indicated for both whiskers. Bottom right, change in curvature (Δκ) for each of the two whiskers. Moments of touch are highlighted. d Single-whisker touch types. e Distribution of curvature changes for each single-whisker touch type. Dark line, mean across mice (n = 7). f Volumetric imaging. Identically colored planes were imaged simultaneously (‘subvolume’ of 3 planes, 20 μm apart; 5-7 subvolumes per mouse). Typical barrel bounds illustrated. g Mean number of trials per subvolume (and, hence, neuron) for each touch type for an example animal (n = 5 subvolumes).