Table 1.

Kinetic parameters of exogenous enzymes in β-ODAP pathway

Enzyme	Organism	Substrate	Km (mM)a	Vmax (×10−4 mM s−1 mg−1 protein)	Vmax/Km (×10−4 s−1 mg−1 protein)
Fpgloxdh	Fomitopsis palustris	Glyoxylate	0.23	0.04	0.17
Scgloxdh (Cyb2p)	Saccharomyces. cerevisiae	Glyoxylate	0.74	0.11	0.15
Pcoah	Penicillium chrysogenum	Oxaloacetate	0.75	7.06	9.41
Fpoah	F. palustris	Oxaloacetate	0.42	3.80	9.05
PanE	Methylobacterium extorquens AM1	Glyoxylate	0.36	14.98	41.61
LsBAHD	Lathyrus sativus	L−2, 3-Diaminopropionate	0.52	0.48	0.92

^aThe experiment was carried out in duplicate. The protein gels and fitting curves are shown in Supplementary Fig. 2, 3, 4, and 6.