Fig. 3. The schematic diagram of transsynaptic study to label all neurons that project onto the NAcSh → VP pathway is presented.

A Retrograde AAV6 was administered to the VP and infected neurons to express TVA, rabies glycoprotein, and mCherry. B Neurons in the NAcSh that express TVA were infected with EnvA pseudotyped rabies virus expressing GFP. Only neurons with TVA can be infected by this pseudotyped rabies. C Rabies replicated in neurons with glycoprotein and transsynaptically “jumps” one synapse in the retrograde direction. D Neurons that express GFP (pseudocolored to magenta) directly synapsed onto NAcSh neurons that project to the VP.