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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Transl Stroke Res. 2022 Mar 20;13(6):1017–1036. doi: 10.1007/s12975-022-01007-6

Fig. 2. Ascorbate Increased 5hmC in Neurons of the Post-Ischemic Cortex.

Fig. 2.

SiRNA (TET3 or Negative control siRNA) was injected 48h before the induction of MCAO. Saline or ascorbate (500 mg/kg; i.p.) was injected at 30 min reperfusion in adult mice following transient MCAO. (A) Increased 5hmC was localized in neurons (NeuN+), (B) but not astrocytes (GFAP+) or (C) microglia (TMEM119+) in the peri-infarct area of the ischemic cortex at 24h reperfusion following transient MCAO (n = 3/group). Scale bar, 15 μm.