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. 2022 Sep 15;3(4):101677. doi: 10.1016/j.xpro.2022.101677
Reagent Final concentration Amount
Neutralized Allyl SAM (∼9 mM) 2.7 mM 6 μL
SUPERase In RNase Inhibitor (20 U/μL) 2 U/μL 2 μL
10 × MjDim1 reaction buffer (400 mM HEPES, pH 8.0, 400 mM NH4Cl and 40 mM MgCl2) 1 × 2 μL
Purified Mjdim1 (1.2–2 mM) 0.4 mM 4 μL
Total N/A 14 μL