Reagent | Final concentration | Amount |
---|---|---|
Neutralized Allyl SAM (∼9 mM) | 2.7 mM | 6 μL |
SUPERase In RNase Inhibitor (20 U/μL) | 2 U/μL | 2 μL |
10 × MjDim1 reaction buffer (400 mM HEPES, pH 8.0, 400 mM NH4Cl and 40 mM MgCl2) | 1 × | 2 μL |
Purified Mjdim1 (1.2–2 mM) | 0.4 mM | 4 μL |
Total | N/A | 14 μL |