TABLE 4.

Pyridine nucleotide content and redox balance for continuous steady-state cultures of C. cellulolyticum

Parameter	Results obtained at D (h−1) ofa:
	0.014	0.027	0.035	0.041	0.055	0.064	0.076	0.083
NADH [μmol (g of cells)−1]	3.39 ± 0.68	4.44 ± 0.89	4.65 ± 0.91	4.79 ± 0.95	4.83 ± 0.98	5.32 ± 1.07	5.68 ± 1.12	6.11 ± 1.19
NAD+ [μmol (g of cells)−1]	8.44 ± 1.73	10.05 ± 2.03	12.08 ± 2.41	13.11 ± 2.64	15.74 ± 2.78	16.16 ± 3.23	16.53 ± 3.32	19.45 ± 3.91
NADPH [μmol (g of cells)−1]	7.47 ± 1.68	8.04 ± 1.89	5.89 ± 1.19	5.65 ± 1.13	4.74 ± 0.94	4.43 ± 0.89	3.82 ± 0.87	4.26 ± 0.95
NADP+ [μmol (g of cells)−1]	0.11 ± 0.03	0.08 ± 0.02	ND	ND	ND	ND	ND	ND
CRC	0.29	0.31	0.28	0.27	0.23	0.25	0.26	0.24
ARC	0.99	0.99	1.00	1.00	1.00	1.00	1.00	1.00
qNADH produced [mmol (g of cells)−1 h−1]	1.94	2.49	2.62	2.74	2.84	2.96	3.15	3.39
qNADH used [mmol (g of cells)−1 h−1]	0.99	1.38	1.55	1.71	1.86	1.99	2.21	2.59
qNADH fd [mmol (g of cells)−1 h−1]	0.94	1.11	1.07	1.03	0.98	0.96	0.94	0.80
H2/CO2 ratio	1.65	1.56	1.58	1.51	1.38	1.41	1.35	1.29
O/R index	0.93	0.95	0.92	0.94	0.99	0.96	0.98	0.97

^aSee Table 2, footnote b. ND, not detectable.