TABLE 6.

Specific enzymatic activity and flux relative to available enzyme activity in C. cellulolyticum cell extract at steady-state growth^a

Enzymea	Results obtained at D (h−1) ofa:
	0.014		0.035		0.064		0.083
	Mean SEA [μmol min−1 (mg of protein)−1] ± SD	R	Mean SEA [μmol min−1 (mg of protein)−1] ± SD	R	Mean SEA [μmol min−1 (mg of protein)−1] ± SD	R	Mean SEA [μmol min−1 (mg of protein)−1] ± SD	R
GAPDH (EC 1.2.1.12)	1.13 ± 0.19		2.37 ± 0.28		2.82 ± 0.31		3.81 ± 0.45
PGM (EC 5.4.2.2)	0.021 ± 0.006	1.3	0.028 ± 0.008	1.2	0.035 ± 0.013	1.1	0.044 ± 0.011	1.3
LDH (EC 1.1.1.27)	0.13 ± 0.01	150.6	0.26 ± 0.03	298.3	0.38 ± 0.04	402.9	0.43 ± 0.05	434.2
PFO (EC 1.2.7.1)	0.44 ± 0.04	10.2	0.57 ± 0.06	9.8	0.71 ± 0.07	10.9	0.74 ± 0.09	9.8
PTA (EC 2.3.1.8)	0.76 ± 0.08	23.9	0.95 ± 0.01	23.2	0.99 ± 0.13	22.8	1.03 ± 0.14	22.1
AK (EC 2.7.2.1)	0.22 ± 0.03	6.9	0.46 ± 0.04	11.3	1.11 ± 0.15	25.5	1.56 ± 0.21	33.6
AADH (EC 1.2.1.10)	0.09 ± 0.01	7.9	0.09 ± 0.02	5.4	0.24 ± 0.02	10.6	0.29 ± 0.03	10.2
ADH (EC 1.1.1.1)	0.17 ± 0.02	15.2	0.25 ± 0.03	14.9	0.41 ± 0.04	18.5	0.49 ± 0.06	17.2

^aSEA, specific activity of enzyme; R, ratio of specific enzymatic activity to metabolic flux through the metabolic pathway (see Results).