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. 2022 Sep 16;219(12):e20220685. doi: 10.1084/jem.20220685

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© 2022 Yang et al.

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Figure 5. — CD47 marks enhanced protein and ATP synthesis and is associated with mitochondria transfer. (A) Representative gating strategy of CD47lo (bottom 30%) and CD47hi (top 30%) fractions within BM and splenic early erythroblasts. (B) Mitochondria transfer frequency in CD47lo and CD47hi splenic and BM early erythroblasts respectively (left), and their relative percentage of occurrence in spleen and BM respectively (right). (C) Mitochondria mass, as determined by mito-Dendra2 fluorescence intensity, of the indicated splenic and BM early erythroblasts populations (CD47lo and CD47hi) under steady state and PHZ stress. (D) ATP content of the indicated splenic and BM early erythroblast populations (CD47lo and CD47hi) under steady state and PHZ stress, measured by CellTiter-Glo Luminescent Cell Viability Assay. (E) Protein synthesis rate, as determined by OP-Puro incorporation, of the indicated splenic and BM early erythroblasts populations (CD47lo and CD47hi) under steady state and PHZ stress. (F) OCR of the indicated splenic and BM early erythroblast populations (CD47lo and CD47hi) under steady state (upper panel) and PHZ stress (lower panel). The quantifications of basal and maximal respiration are shown. For all quantification, mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P ≤ 0.001 by Student’s t test.