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. 2022 Sep 16;219(12):e20220685. doi: 10.1084/jem.20220685

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© 2022 Yang et al.

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Figure S5. — In vitro validation of mitochondria transfer functions and characterization in bleeding model. (A) Schematic illustration of the experiment to analyze the functions of mitochondria transfer from in vitro culture of splenic early erythroblasts (mito−: w/o mito-transfer; and mito+: mito-transfer). (B) Proliferation of the indicated splenic early erythroblasts populations measured by CellTiter-Glo Luminescent Cell Viability Assay. (C) FACS analysis of reticulocytes/RBC fractions from in vitro culture of mito− and mito+ early erythroblasts respectively. For all quantification, mean ± SEM; *, P < 0.05; **, P < 0.01, by Student’s t test. (D) Cd47 mRNA expression by gene expression microarray on BM-HSPC, mature leukocytes, stromal cells and nucleated erythroid cells. Data are from the Gene Expression Commons dataset (https://gexc.riken.jp/models/1649/genes/Cd47). (E) Sirpα mRNA expression by gene expression microarray on BM-HSPC, mature leukocytes, stromal cells and nucleated erythroid cells. Data were obtained from the Gene Expression Commons dataset that is available online (https://gexc.riken.jp/models/1649/genes/Sirpa). (F) Representative flow cytometric plots showing the gating of mito-Dendra2⁺ population that were identified as having received mitochondria in BM and splenic early erythroblasts after bleeding.