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Actual and coded levels of the independent variables of the Box–Behnken design (BBD). TIC, total inorganic carbon; N:P, nitrogen-to-phosphorus ratio; TP, total phosphorous.
| Independent variables | Symbol | Experimental Values | ||
|---|---|---|---|---|
| Low (-1) | Central (0) | High (+1) | ||
| TIC (mM) | A | 1.8 | 5.4 | 9 |
| N:P ratio (TP: 10 mg L−1) | B | 5 | 10 | 15 |
| Light Intensity (μE) | C | 100 | 300 | 500 |
