TABLE 3.
Matrix | Intra-batch (n = 6) | Inter-batch (n = 6 × 3) | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
LLOQ QC | LQC | MQC | HQC | LLOQ QC | LQC | MQC | HQC | |||||||||
A | P | A | P | A | P | A | P | A | P | A | P | A | P | A | P | |
Cryopreserved human whole blood | −2.4 | 8.0 | −0.7 | 12.8 | −2.7 | 6.8 | 4.3 | 7.7 | −4.0 | 8.8 | −11.3 | 10.5 | 1.3 | 7.9 | 5.7 | 8.5 |
Fresh human whole blood | 11.8 | 2.9 | 4.0 | 6.4 | 0.0 | 6.7 | 3.0 | 9.0 | 5.0 | 13.0 | 2.0 | 10.5 | 0.0 | 7.3 | 6.8 | 7.3 |
Note: A, accuracy and data are expressed as relative error (RE, %); P, precision, and data are expressed as the relative standard deviation (RSD, %); n, number of replicates; LLQC, 0.500 ng/ml; LQC, 1.50 ng/ml; MQC, 15.0 ng/ml; HQC, 40.0 ng/ml.