TABLE 4.
Recovery and matrix effect of SRL in cryopreserved and fresh human whole blood.
| Nominal conc. (ng/ml) | Recovery (n = 6) | IS-normalized matrix factor (n = 3 × 6) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cryopreserved human whole blood | Fresh human whole blood | Cryopreserved human whole blood | Fresh human whole blood | |||||||||
| Mean (%) | RSD (%) | Total RSD (%) | Mean (%) | RSD (%) | Total RSD (%) | Mean ± SD (%) | RSD (%) | Total RSD (%) | Mean ± SD (%) | RSD (%) | Total RSD (%) | |
| 1.50 | 88.6 | 5.6 | 4.7 | 96.7 | 12.6 | 9.4 | 112.2 ± 7.6 | 6.8 | 4.2 | 109.0 ± 3.1 | 2.8 | 3.1 |
| 15.0 | 81.0 | 4.8 | 80.7 | 6.4 | 103.8 ± 4.1 | 3.9 | 102.6 ± 3.3 | 3.2 | ||||
| 40.0 | 87.2 | 6.1 | 93.7 | 12.1 | 111.1 ± 8.0 | 7.2 | 104.8 ± 3.5 | 3.3 | ||||
Note: RSD, relative standard deviation; total RSD, the RSD for three concentration levels; n, number of replicates.