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. 2022 Sep 5;65:101590. doi: 10.1016/j.molmet.2022.101590

Figure 8.

Figure 8

Vagal afferent GLP-1Rs are sensitive to diet-induced dyslipidemia.A-H: Syrian golden hamsters were fed a high fructose diet or chow control for 2-weeks. Hamsters were then fasted for 16 h before receiving portal vein injections of either GLP-1(7-36) (10 μg/kg) or vehicle (PBS). I-Q: Alternatively, hamsters were fed a fat/fructose/cholesterol enriched diet (FFC) for 4 weeks. Hamsters were then fasted for 16 h before receiving bilateral nodose ganglia injections of GLP-1(7-36) (1ug in 1 μl PBS) or vehicle (1 μl PBS). All hamsters were then fat loaded with an oral gavage of olive oil (200 μl) and then received IV tyloxapol (2  g/kg; A-D) or an IP injection of Pluronic F-127 (2  g/kg; I-L). Blood was then drawn over a 6 h period via jugular catheter (A-D) or retro-orbital bleeds (I-L). (A/I) 2/4-week fasting plasma TG, (B/K) food consumption, (C/L) weight, (D/M) body weight change, (J) 4-week fasting plasma cholesterol, (E/N) plasma TG, (F/O) plasma TG AUC, (G/P) TRL TG, (H/Q) TRL TG AUC. Data is presented as means ± SEM (A-H: n=6–7/group; I-Q: n=6–7/group). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.0001). ∗; Chow Vehicle vs. Chow GLP-1, ˆ; Chow GLP-1 vs. Fructose/FFC Vehicle, $; Chow GLP-1 vs. Fructose/FFC GLP-1.