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Identification of cytokeratin 14 (K14, green), cytokeratin 6 (K6, red), collagen IV α2 (COL4α2, blue), collagen IV α3 (COL4α3, blue) and collagen IV α6 (COL4α6, blue) in the human Meibomian gland (MG). K14 is present in both ductal and acinar epithelial cells, whereas K6 expression is restricted to ductal cells. Human MG tissues stain positive for COL4α2 (A) and COL4α6 (C), but negative for COL4α3 (B). Scale bar = 100 μM.
