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. 2022 Sep 6;9:981610. doi: 10.3389/fmed.2022.981610

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Copyright © 2022 Chen, Chen, Xie, Hatton, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of K14 (green), glycoproteins (red) and proteoglycans (red) in the human Meibomian gland (MG). Nuclei were counterstained with 4', 6-Diamidino-2-phenylindole (DAPI) in blue. Laminin α2 (A), laminin β1 (B) and perlecan (D) stained positive surrounding the acini and the acinar/ductal junction, but not the region around the duct. Tenascin-C (C) was found specifically located at the junctions between the acini and the central duct (white arrows). The staining was negative for both agrin (E) and endostatin (F) in the human MG tissues. Scale bar = 100 μM.