FIG. 1.

(A) Schematic representation and physical map of the hrcA-dnaK operon. The CIRCE element and the hrcA gene are indicated with a hairpin and thick arrow, respectively. A 1.1-kbp PCR product obtained from primers HF and HR, the EcoRI fragment cloned in pTY-1, and the BamHI fragment in pETTY are shown as bars, as are pTI-2 (26) containing the C terminus of the hrcA gene with the grpE, dnaK, and dnaJ genes. (B) DNA sequence of the N terminus of the hrcA gene and its 5′-flanking region. The vegetative promoter (−35 and −10 consensus sequence) is boxed. The CIRCE element is indicated with thick arrows. A Shine-Dalgarno sequence (SD) for ribosome binding is underlined.