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. 2021 Jul 24;45(4):642–653. doi: 10.1016/j.bj.2021.07.005

Fig. 2.

Fig. 2

The effect of overexpression of OIP5-AS1 on the cell viability and apoptosis of cisplatin-stimulated HK-2 cells. (A) The transfection efficiency of pc-DNA-OIP5-AS1 and pcDNA-vector was evaluated by qRT-PCR. (B) HK-2 cells were transfected with pc-DNA-OIP5-AS1 and pcDNA-vector, then induced with cisplatin for 24, 48, 72 and 96 h. Cell viability was evaluated by CCK-8 kit. (C–E) The HK-2 cells were transfected with pc-DNA-OIP5-AS1 and pcDNA-vector, then induced with or with cisplatin for 24 h. (C) The apoptosis rate was evaluated by flow cytometry. (D) The protein expression levels of apoptosis-related makers C-caspase 3 and C-caspase 9 were detected by Western blot. (E) The protein expression levels of Bax and Bcl-2 were evaluated by Western blot. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. pcDNA-vector (−) CP group.