Fig. 4.

MiR-144-5p mimics significantly reversed the effects of overexpression of OIP5-AS1 on cell viability and apoptosis in cisplatin-stimulated HK-2 cells. HK-2 cells were transfected with pcDNA-OIP5-AS1, or ac-transfected with pcDNA-OIP5-AS1 and miR-144-5p mimics, then cells were treated with or without cisplatin. (A) Cell viability for different times was evaluated by CCK-8 kit. (B) The apoptosis rate for 24 h was evaluated by flow cytometry. (C) The protein expression of C-caspase 3 and C-caspase 9 for 24 h was detected by Western blot. (D) The protein expression levels of Bax and Bcl-2 for 24 h were detected by Western blot. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. control group without cisplatin. ^##p < 0.01 vs. pcDNA-OIP5-AS1 group with cisplatin.