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. 2022 Aug 13;298(10):102375. doi: 10.1016/j.jbc.2022.102375

Table 3.

Steady-state kinetics parameters for SmHDAC8 WT and hHDAC8

Enzyme	SmHDAC8			hHDAC8^§
Conditions	Ctrl (DMSO)∗	5μM NF2886#	1μM NF2889∗	Ctrl (DMSO)	5μM NF2886	2μM NF2889
k_cat (s⁻¹)	0.017 ± 0.004	0.017 ± 0.003	0.007 ± 0.003	0.010 ± 0.001	0.012 ± 0.004	0.011 ± 0.005
K_M (μM)	737 ± 197	2403 ± 1590	273 ± 163	302 ± 4.5	1119.5 ± 70.5	662.5 ± 53.5
k_cat/K_M	22.8	7.2	25.6	31.4	10.7	15.8

Average of four (∗), three (#), or two (^§) independent experiments ± SD (for SmHDAC8, statistical significance of NF2889 versus Ctrl, p < 0.05).