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. 2022 Sep 6;10:1006374. doi: 10.3389/fbioe.2022.1006374

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Copyright © 2022 Wang, Zhang, Yang, Shi, Li, Yu, Wei and Ding.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Response of MBs-Apt₆₃ to UV in vitro and identification of exosomes (A) Agarose gel electrophoresis (molecular weight of free aptamer = 32 bp) of MBs-Apt₆₃ at different concentrations after UV exposure; (B) Agarose gel electrophoresis of MBs-Apt₆₃ (10.0 μg/ml) after exposure to UV for different times (molecular weight of free aptamer = 32 bp). (C) The expression of CD9, CD63 and calnexin determined by western blotting; (D) The particle size of breast cancer cell exosomes characterized by DLS; (E,F) Transmission electron microscopy image of breast cancer exosomes (magnification = 50000× in C and 100000× in D, exosomes are shown in the red dotted circle).