TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or phenotypea | Source or reference |
|---|---|---|
| V. cholerae | ||
| O395 | Wild type (classical Ogawa); Smr | 36 |
| C6709 | Wild type (El Tor Inaba); Smr | 5 |
| JB29 | O395 lacZ::toxT-gusA; Smr | This study |
| JB62 | JB29 eno::TnphoA; Smr Kmr | This study |
| JB65 | JB29 rhlB::TnphoA; Smr Kmr | This study |
| JB66 | JB29 cadB::TnphoA; Smr Kmr | This study |
| JB69 | JB29 pepA::TnphoA; Smr Kmr | This study |
| JB70 | JB29 yhdA::TnphoA; Smr Kmr | This study |
| JB73 | JB29 argH::TnphoA; Smr Kmr | This study |
| JB98 | JB29 pepA::pJB10; Smr Apr | This study |
| JB104 | YM2-34 pepA::pJB10; Smr Apr | This study |
| JB115 | JB29 lap::pJB12; Smr Apr | This study |
| JB123 | JB29 ΔpepA; Smr | This study |
| JB129 | JB123 toxR::pVM55; Smr Apr | This study |
| JB131 | JB123 tcpP::pYM2-16; Smr Apr | This study |
| JB132 | YM2-34 ΔpepA; Smr | This study |
| JB134 | JB29 toxR::pVM55; Smr Apr | This study |
| JB135 | JB29 tcpP::pYM2-16; Smr Apr | This study |
| JB148 | YM2-34 aphB::pJB15; Smr Apr | This study |
| JB150 | JB132 aphB::pJB15; Smr Apr | This study |
| JB152 | JB123 ΔpepA::pJB14; Smr Apr | This study |
| YM2-34 | O395 lacZ::tcpP-gusA; Smr | 45 |
| E. coli | ||
| DH5α | F−endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 (φ80dlacZΔM15) | 20 |
| DH5α λpir | λpir lysogen of DH5α | Laboratory strain |
| SM10 λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir R6K | 43 |
| Plasmids | ||
| pUJ10 | Plasmid for the construction of divergent fusions to lacZ/phoA reporters; Apr | 12 |
| pGP704 | Suicide vector; pBR322 derivative with oriR6K mobRP4; Apr | 19 |
| pT689 | A p6891MCS derivative which contains the rrnB transcriptional terminator from plasmid pKK223-3; Apr | 45 |
| pCVD442 | Positive selection suicide vector; Apr | 16 |
| pYM2-16 | pGP704 derivative carrying a 230-bp internal tcpP fragment; Apr | Y. M. Murley and S. B. Calderwood, unpublished data |
| pVM55 | Suicide plasmid pJM703.1, containing an internal fragment of toxR; Apr | 43 |
| pJB4 | pUJ10 derivative carrying the toxT-gusA fusion; Apr | This study |
| pJB6 | pT689 derivative carrying the toxT-gusA fusion; Apr | This study |
| pJB10 | pGP704 derivative carrying an internal pepA fragment; Apr | This study |
| pJB12 | pGP704 derivative carrying an 800-bp internal fragment of lap; Apr | This study |
| pJB13 | pCVD442 derivative carrying a pepA allele containing an in-frame deletion; Apr | This study |
| pJB14 | pGP704 derivative carrying a wild-type copy of pepA; Apr | This study |
| pJB15 | pGP704 derivative carrying a 584-bp internal fragment of aphB; Apr | This study |
a
Sm, streptomycin; Ap, ampicillin; Km, kanamycin; Tc, tetracycline.