TABLE 2.

NOR activities of membranes prepared from P. denitrificans GB1 grown under different conditions with different plasmids

Plasmid present	NOR expresseda	Growth condition	NOR activity (nmol · mg of protein−1 · min−1)
None	None	O2, succinate	<5
None	None	O2, methylamine	<5
None	None	O2, L broth	<5
pCYCNOR3	WT	O2, succinate	23
pCYCNOR3	WT	O2, methylamine	230
pCYCNOR3	WT	O2, L broth	300
pCYCNOR3	WT	Anaerobic, succinate + nitrate	440
p125CNOR	E125A	O2, methylamine	<5
p198CNOR	E198A	O2, methylamine	<5
P202CNOR	E202A	O2, methylamine	200
p198202CNOR	E198A/E202A	O2, methylamine	<5
p125CNOR	E125A	O2, L broth	<5
p198CNOR	E198A	O2, L broth	<5
P202CNOR	E202A	O2, L broth	210
p198202CNOR	E198A/E202A	O2, L broth	<5

^aWT, wild type; E125A, NOR^E125A (similar for E198A and E202A).