TABLE 3.
NOR and oxidase activities in membranes prepared from E. coli JM109 expressing wild-type or mutant forms of NOR
| Plasmids present | NOR expresseda | NOR activity (nmol of NO mg of protein−1 min−1) | Oxidase activity (nmol of O mg of protein−1 min−1) |
|---|---|---|---|
| None | None | <5 | |
| pEC86 | None | <5 | |
| pEC86, pNOREX | Wild type | 470 | 40 |
| pEC86, p125EX | E125A | 20 | 2 |
| pEC86, p198EX | E198A | <5 | <1 |
| pEC86, p202EX | E202A | 140 | 30 |
| pEC86, p198202EX | E198A/E202A | <5 | <1 |
E125A, NORE125A (similar for E198A and E202A).