Figure 3.

IRI results in a higher degree of glycolytic stress in HFD mice. (A) Color mapped Phasor FLIM images of the ND and HFD liver samples in the absence (left) and presence (right) of NAC supplementation. The top row shows the sham samples, and the bottom row shows the IRI samples. More green, blue and red color represents more protein-bound NADH, free NADH and long lifetime species (LLS), respectively. (B) Phasor plot of autofluorescence FLIM in liver samples – the three colored circles represent the lifetime position of the three components: free and bound NADH, and LLS. (C) The concentration ratio of free to bound NADH was calculated from each mouse. The higher value represents more glycolytic stress. There is a higher level of glycolytic stress in HFD and HFD (IRI) samples. This is reduced in the HFD + NAC (IRI) samples, but not the HFD + NAC samples, respective to their HFD counterparts. (D) The fractional intensity of LLS calculated using three-component calculations to show the increasing LLS in high fat liver and how that increases with IRI (n=5-7 mice per group for both analyses). “*” 0.05 to 0.01.