Fig. 1.

Reduced MPST expression was found in the intestine of patients with IBD and in chemically induced mouse acute experimental colitis. (A) Representative images of immunohistochemical staining of intestinal sections from the controls and the patients with UC and CD. (B) Boxplot of MPST expression levels in endoscopic-derived intestinal mucosal biopsies from patients with CD, patients with UC and healthy controls (using dataset GSE59071; control, n = 11; CD, n = 8; UC, n = 97). (C) The expression of MPST in intestinal tissues from the controls and the patients with UC was assessed via Western blots, and densitometry analysis is shown. (D) Western blotting and densitometry analysis of MPST protein expression in colons from mice treated with or without 3.5% DSS for 7 days. (E) Intestinal MPST mRNA expression in normal mice (n = 4) and mice treated with 3.5% DSS (n = 5) for 7 days was determined by quantitative PCR. (F) Western blotting and densitometry analysis of MPST protein in IECs isolated from the controls and the 3.5% DSS-treated mice. (G and H) MPST mRNA (G) and protein expression (H) in HT29 cells treated with or without TNF-α. (I) Intestinal stem cells were harvested from untreated WT mice, and the organoids were stimulated with mouse recombinant TNF-α for 24 h starting on Day 6. Western blotting and densitometry analysis of MPST protein in organoids are shown. Fig. 1C, D, Fig. 1F, H and I were repeated three times on three different experimental days. Three to four replicates were used in Fig. 1C, D and Fig. 1, Fig. 2 replicates were used in Fig. 1H and I. Values are expressed as the mean ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001.