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. 2022 Sep 11;56:102469. doi: 10.1016/j.redox.2022.102469

Fig. 2.

Fig. 2

MPST deficiency markedly aggravates murine colitis. (A–H) MPST+/+ (n = 7), MPST+/− (n = 7), and MPST−/− (n = 7) mice were treated with 3.5% DSS for 6 days. (A–C) Body weight (A) and the disease activity index (B) calculated from weight loss, stool consistency, and bleeding were scored daily. Mice were sacrificed on day 6, and colon length (C) was measured. (D) Mice were fed FITC-dextran (60 mg/100 g) 4 h before sacrifice on day 6, and the serum level of FITC-dextran was detected with a microplate reader. (E) Histological changes in colon tissue samples from DSS-treated MPST+/+ mice (MPST+/+ DSS), DSS-treated MPST +/− mice (MPST +/− DSS) and DSS-treated MPST−/− mice (MPST−/− DSS) were examined by hematoxylin and eosin staining (original magnification, × 50). (F) Semiquantitative scoring of histopathology. (G) The relative mRNA levels of cytokines and chemokines in the colon were calculated (TNF-α, IL-1β, IL-6 and CCL3). (H) Western blotting was used to analyze iNOS, p-Iκbα (Ser32), p-p65 (Ser 536) and MPST protein expression in colons harvested from mice after DSS administration. Values are expressed as the mean ± S.D. in this figure. The WB was repeated three times on three different experimental days. Three to four replicates were used in Fig. 2H.