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MPST deficiency inhibited stem-cell-derived organoid formation (A–B) Western blotting and IHC were used to analyze MPST protein expression in organoids harvested from MPST+/+, MPST +/- and MPST−/− mice. (C) Intestinal stem cells were harvested from untreated MPST+/+, MPST +/- and MPST−/− mice, and the organoids were observed daily. (D) Organoids were stimulated with mouse recombinant TNF-α for 24 h on Day 7.
