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. 2022 Sep 11;56:102469. doi: 10.1016/j.redox.2022.102469

Fig. 5.

Fig. 5

MPST deficiency markedly aggravates cellular inflammation (A) mRNA expression of proinflammatory cytokines and chemokines (TNF-α, ICAM, CXCL5 and CX3CL1) after MPST knockdown in TNF-α-treated HT29 cells (n = 3/per group). (B) mRNA expression of proinflammatory cytokines and chemokines (TNF-α, ICAM, CXCL5 and CX3CL1) after MPST overexpression in TNF-α-treated HT29 cells (n = 3/per group). (C) ROS levels after MPST overexpression in TNF-α-treated HT29 cells (n = 6/per group). (D) ROS levels after MPST knockdown in TNF-α-treated HT29 cells (n = 3/per group). (E) Western blotting was used to analyze NF-κb protein expression in MPST-deficient HT29 cells. Values are expressed as the mean ± S.D. in this figure. This WB was repeated three times (2 replicates were used each) on three different experimental days. *, p < 0.05; **, p < 0.01; ***, p < 0.001.