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Electronic spectra of n-FY6 OaPAC Variants in H2O. Comparing the dark (A) and light (B) state absorbance spectra of n-FY6 variants to wild-type OaPAC, the flavin electronic state is not perturbed by the fluorotyrosine. Only 3-FY6 and 2,3-F2Y6 form a red-shifted spectrum upon illumination of the sample with 450 nm LED, and both have a difference electronic spectrum illustrating an absorbance of the light state at 493 nm.
