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. Author manuscript; available in PMC: 2023 Sep 1.
Published in final edited form as: Mol Microbiol. 2022 Aug 15;118(3):125–144. doi: 10.1111/mmi.14968

Figure 9. d- and l-serine catabolism both contribute to P. mirabilis biofilm formation in LB but not in human urine.

Figure 9.

Wild-type P. mirabilis and indicated mutants were suspended in either LB (A-D) or filter-sterilized pooled human urine (E) and distributed into 24-well plates. After a 20 hour incubated at 37°C under stationary conditions, biofilm biomass was quantified by crystal violet staining (A, C, D, and E) or by dilution and plating for determination of CFUs/ml (B). (C) LB was supplemented with 10 mM l-serine. (D) LB was supplemented with 10 mM alanine or pyruvate. Error bars represent mean ± SD for at least three independent experiments with at least 2 replicates each. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Dunnett’s test for multiple comparison. ns=not significant.