Table 2:
Summary of phenotypes associated with d vs l serine utilization
| Phenotypes | ΔdsdA | ΔsdaAB | ΔsdaABΔdsdA |
|---|---|---|---|
| Growth in PMSM | +/− | +/− | +/− |
| Ability to use d-serine as sole C or N source | --- | - | --- |
| Complementation: | pdsdA | NA | pdsdA |
| Ability to use l-serine as sole C or N source | +/− | --- | --- |
| Complementation: | NA | psdaA or psdaB | psdaA or psdaB |
| Growth in LB | +/− | +/− | +/− |
| Relative fitness in LB | +/− | - | -- |
| Complementation: | NA | Neither alanine nor pyruvate | Neither alanine nor pyruvate |
| Probable causes: | NA | Membrane potential, AA homeostasis | Membrane potential, AA homeostasis |
| Growth in urine | +/− | +/− | +/− |
| Relative fitness in urine | +/− | - | -- |
| Complementation: | NA | psdaA or psdaB, but not alanine or pyruvate | psdaA or psdaB, but not alanine or pyruvate |
| Probable causes: | NA | Membrane potential, AA homeostasis | Membrane potential, AA homeostasis |
| Antibiotic/detergent sensitivity | +/− | +/− | -- |
| Complementation: | NA | NA | Neither alanine nor pyruvate |
| Probable causes: | NA | Membrane potential, AA homeostasis | Membrane potential, AA homeostasis |
| Membrane permeability | +/− | -- | --- |
| Swarming motility | +/− | - | --- |
| Complementation: | NA | psdaA, psdaB, or alanine | Partially by pdsdA, fully by psdaA, psdaB, or alanine |
| Probable causes: | NA | Cell wall perturbation | Cell wall perturbation |
| Swimming motility | +/− | -- | --- |
| Complementation: | NA | Partially by alanine, fully by psdaA or psdaB, | Partially by psdaB or alanine, fully by psdaA |
| Probable causes: | NA | Cell wall perturbation, membrane potential, AA homeostasis | Cell wall perturbation, membrane potential, AA homeostasis |
| Biofilm formation in LB | - | -- | -- |
| Complementation: | l-serine | Neither alanine nor pyruvate | Neither alanine nor pyruvate |
| Probable causes: | Perturbation of l-serine synthesis | Membrane potential, AA homeostasis | Membrane potential, AA homeostasis |
| Biofilm formation in urine | +/− | +/− | +/− |
| CAUTI colonization | - | --- | --- |
| CAUTI relative fitness | --- | --- | --- |
(+/−) similar to wild-type; (-) slight defect; (--) moderate defect; (---) severe defect; NA not applicable