TABLE 1.
Effects of Δ(T · A)n mutations on upp::lacZ expression and regulationa
| Strain (T · A tract) | β-Galactosidase activity (nmol/min/mg)b
|
Fold regulation | |
|---|---|---|---|
| Uracil | UMP | ||
| CLT5178 (T8 [wild type]) | 1,580 | 9,020 | 5.71 |
| CLT5249 (T7) | 1,570 | 8,610 | 5.48 |
| CLT5248 (T6) | 3,200 | 12,700 | 3.97 |
| CLT5247 (T5) | 4,540 | 15,300 | 3.37 |
| CLT5246 (T4) | 5,690 | 13,900 | 2.44 |
| CLT5245 (T3) | 6,990 | 10,300 | 1.47 |
| CLT5218 (T2) | 5,940 | 9,030 | 1.52 |
a
Doubling times were 47 ± 1 min for cells grown on uracil and 66 ± 2 min for cells grown on UMP.
b
Means of six experiments with standard deviations of ≤7%.