Table 3.

Effect of ergot alkaloids (EA) and a mycotoxin deactivating product (MDP) on pH, total gas, and methane (CH₄) production when fed a high grain diet in the RUSITEC

Item	Control		EA		SEM	P-value
	-MDP1	+MDP2	-MDP	+MDP		EA	MDP	EA × MDP
pH	6.62	6.61	6.61	6.60	0.014	0.35	0.30	0.91
Total gas, litersd	2.99	3.01	3.00	3.18	0.292	0.57	0.51	0.59
CH4, %3	4.46ab	4.80a	4.54ab	3.70b	0.454	0.04	0.30	0.02
CH4, mL/d	129	141	138	121	14.3	0.66	0.85	0.31
CH4, mg/d	85.6	93.2	90.9	80.0	9.45	0.66	0.85	0.31
CH4, mg/g of OM fermented	10.14	11.65	10.84	9.25	1.14	0.45	0.97	0.17

Within a row, means without a common superscript difference (P < 0.05).

-MDP, diet without mycotoxin deactivating product.

+MDP, diet with mycotoxin deactivating product: 1g of MDP added to 10 g of diet DM, containing mineral adsorbents and dried yeast, fed at 1g/vessel per day.

Percentage of total gas