Table 5.

Effects of ergot alkaloids (EA) and a mycotoxin deactivating product (MDP) on ammonia and volatile fatty acid production in RUSITEC fermenters fed a high grain diet

Item	Control		EA		SEM	P-value
	-MDP1	+MDP2	-MDP	+MDP		EA	MDP	EA × MDP
NH3-N3, mg/d	67.9	72.7	65.9	73.6	5.10	0.90	0.15	0.72
Total VFA4, mmol/d	63.2	67.4	60.6	67.3	2.70	0.32	<0.001	0.35
VFA proportions, mol/100 mol
Acetate (C2)	47.7	48.4	45.6	47.4	0.97	0.01	0.03	0.29
Propionate (C3)	25.6	25.9	24.7	27.0	1.12	0.85	0.03	0.09
Butyrate	15.6	16.0	17.3	15.8	1.23	0.13	0.30	0.06
Valerate	5.45	4.53	6.17	4.72	0.574	0.09	<0.001	0.32
Isovalerate	4.60	4.05	5.19	4.60	0.393	0.03	0.04	0.95
Isobutyrate	0.78	0.81	0.75	0.79	0.263	0.33	0.08	0.88
Caproate	0.32	0.16	0.27	0.14	0.058	0.41	0.002	0.75
C2/C35	1.89	1.86	1.81	1.75	0.071	0.03	0.28	0.77

-MDP, diet without mycotoxin deactivating product.

+MDP, diet with mycotoxin deactivating product: 1g of MDP added to 10 g of diet DM, containing mineral adsorbents and dried yeast, fed at 1g/vessel per day.

NH₃-N, ammonia nitrogen.

VFA, volatile fatty acid.

C2/C3, acetate:propionate ratio.