Table 7.
Effect of ergot alkaloids (EA) and a mycotoxin deactivating product (MDP) on microbial diversity indexes, the abundance of archaea and bacteria, and phylum-level taxonomic composition of the most abundant bacterial communities (%) in the RUSITEC when fed a high grain diet (n = 4 per treatment)
| Item | Control | EA | SEM | P-value | ||||
|---|---|---|---|---|---|---|---|---|
| -MDP1 | +MDP2 | -MDP | +MDP | EA | MDP | EA × MDP | ||
| Shannon Diversity Index | 6.5 | 6.6 | 6.4 | 6.4 | 0.07 | 0.05 | 0.09 | 0.60 |
| Phylogenetic Diversity | 26.3 | 27.8 | 27.1 | 27.3 | 0.68 | 0.82 | 0.25 | 0.40 |
| Archaea | 2.3 | 2.3 | 2.3 | 2.4 | 0.99 | 0.85 | 0.89 | 0.91 |
| Bacteria | 97.7 | 97.7 | 97.7 | 97.6 | 0.99 | 0.85 | 0.89 | 0.91 |
| Firmicutes | 50.4 | 42.0 | 49.7 | 47.0 | 3.74 | 0.56 | 0.15 | 0.45 |
| Bacteroidota | 24.8 | 31.6 | 26.2 | 29.5 | 3.45 | 0.88 | 0.04 | 0.42 |
| Proteobacteria | 8.6 | 7.8 | 9.5 | 9.3 | 2.03 | 0.54 | 0.79 | 0.87 |
| Actinobacteriota | 3.6 | 5.5 | 2.7 | 4.0 | 0.72 | 0.09 | 0.04 | 0.65 |
| Spirochaetota | 4.0 | 3.6 | 3.5 | 2.3 | 1.70 | 0.20 | 0.27 | 0.57 |
| Synergistota | 3.8 | 2.7 | 3.0 | 2.6 | 0.76 | 0.40 | 0.12 | 0.49 |
| Verrucomicrobiota | 2.1 | 2.2 | 1.4 | 1.4 | 0.46 | 0.05 | 0.87 | 0.87 |
| Planctomycetota | 0.9 | 1.6 | 1.4 | 2.0 | 0.20 | 0.05 | 0.01 | 0.92 |
| Others (<0.5%) | 1.9 | 2.5 | 1.9 | 2.1 | 0.5617 | 0.68 | 0.56 | 0.61 |
-MDP, diet without mycotoxin deactivating product.
+MDP, diet with mycotoxin deactivating product: 1g of MDP added to 10 g of diet DM, containing mineral adsorbents and dried yeast, fed at 1g/vessel per day.