Table 1. Strains Used in This Study.
| name | relevant features | reference |
|---|---|---|
| E. coli MG1655 | K-12 F– λ– | (63) |
| E. coli DB3.1 | F–gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB–, mB–) ara14 galK2 lacY1 proA2 rpsL20(StrR) xyl5 Δleu mtl1 | Invitrogen |
| E. coli Top10 | F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ– | Invitrogen |
| ΔacrA | MG1655 ΔacrA::cat, CmR | this work |
| ΔacrAB | MG1655 ΔacrAB::cat, CmR | this work |
| ΔrybB | MG1655 ΔrybB::cat, CmR | this work |
| acrA–SD–syfp2 | MG1655 acrA-SD-syfp2-cat, transcriptional fusion, CmR | this work |
| acrA-9′–syfp2 | MG1655 acrA-9′-syfp2-cat, translational fusion of first 9 acrA codons, CmR | this work |